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Sulfur (sulfur according to KHP2008) is mainly composed of sulfur [S]. As a Korean traditional medicine,Sulfur has an effect on destroying insects and treatment of constipation. However, Sulfur that is distributed domestically and overseas has unclear origin, and its safety is not guaranteed. In this study, we established the criteria of sulfur standard as a medicine. 14 samples were obtained from domestic and oversea markets. Major element, sulfur, was measured by X-ray fluorescence spectrometer(XRF) and minor toxic elements such as As,Cd, Pb were determined by ICP/OES. Hg was determined by mercury analyzer. Most of samples, except S-10 and S-11, were proven to be high quality genuine sulfurs. S-10 and S-11 contains high concentration of As and presumed to be realgar.