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This study explores the effects of physical activity on the HRV and stress level among female high schoolstudents. We measured the physical activity level of two hundred students with the International Physical ActivityQuestionnaire. Physical activity was categorized as vigorous, moderate, and walking. We selected twenty students foreach category and measured their HRV. By using the IEMBIO, canopy9 and program PASW Statistics18, the collecteddata calculated the average value of the measurement items and the standard deviation was statistically treated asfollows. The results of this study are as follows; 1) There was no difference in BMI among the three groups. 2) Therewas a little difference of stress among the three groups. 3) There was a little difference in the average heart ratebetween the three groups on the degree of participation in physical activity. 4) There was no difference in RMSSDbetween the three groups on the degree of participation in physical activity. 5) There was no difference in TP betweenthe three groups on the degree of participation in physical activity. 6) There was a difference in LF between the threegroups on the degree of participation in physical activity. And LF of vigorous physical activity group was higher thanwalking physical activity group. These results suggest that higher physical activity level is associated with higher heartrate stability, sympathetic nervous activity, and stress resistance.