목적: 조기분만의 발병원인으로 자궁내감염증을 포함한 다양한 태반융모세포내 면역학적 변화를 알아보기 위하여 자가면역항 체로 알려진 hsp60 및 hsp27의 발현 여부를 통해서 hsp의 세포보호작용 (cytoprotective)에 대하여 알아보고자 하였다 방법: 24~36주 사이에 조기분만한 22예는 자연 발생한 조기진통과 파수 24시간 이내의 경우만을 포함하였고 산모 또는 태아의 원인으로 인한 조기분만은 제외하였다. 한편 대조군으로 만삭분만한 22예의 산모는 진통 시 발생하는 스트레스유발 가능성 을 배제하기 위하여 반복제왕절개를 받은 산모의 태반융모세포만을 취하였다 각각의 태반조직에서 추출한 태반융모세포로 부터 단백질을 추출하여 정량한 다음, 형광현미경으로 두 군 사이의 hsp60 및 hsp27의 존재 유무 및 그 차이를 살펴보았다. 또한 Western blots을 이용하여 나타나는 단백질 발현의 세기를 영상판독기를 이용하여 두 군 사이의 변화를 비교하였다. 결과: hsp60과 hsp27은 조기분만의 태반융모세포 및 만삭분만의 태반융모세포에서 모두 관찰되었다. 자가면역항체의 한 형태 로 생각되는 hsp60은 조기분만의 태반융모세포에서 만삭분만의 태반융모세포보다 더 강하게 나타나는 것을 확인 하였으며 (P<0.001), 태반융모세포에서 특이적으로 발현되는 것으로 알려진 hsp27도 조기분만의 태반융모세포에서 더 강하게 나타났 다 (P<0.02). 결론: 조기분만에 관여하는 많은 요인들로 인하여 세포에 많은 스트레스 유발 작용이 존재함을 확인할 수 있었으며 hsp 이외에 다른 단백질과의 상호 작용 등에 대한 검증이 필요할 것으로 사료된다.

Objective: To compare the distribution and expression of hsp60 and hsp27 in placental trophoblast between preterm and term placenta and to observe hsp immune response in relation to the pathogenesis of preterm birth. Methods: 22 cases of preterm trophoblast, between 24 weeks and 36 weeks gestation, which were developed spontaneous onset or less than 24 hours after rupture of membrane were obtained. And aged-matched, 22 cases of normal term trophoblast, as control were also obtained after informed consent from each patient. The protein extraction form trophoblast was stained by immunohistochemical methods and was measured by the assay of Western blots. And the density of band using Image-writer were taken and statistical assay were performed as significance <0.05. Results: The expressions of hsp60 and hsp27 in trophoblast of preterm and term placenta were identified by immunohistochemical staining method. The hsp60 had significantly higher expression in trophoblast of preterm birth than in that of term birth (P<0.001) and the hsp27 also had significantly higher expression in trophoblast of preterm birth than in that of term birth (P<0.02) Conclusion: The higher expression of hsp60 and hsp27 in trophoblast of preterm birth might be suggested the development of immune response to occur preterm labor Further study are necessary to determine the exact actions of hsp60 and27 in trophoblast and to understand the immune mechanism of preterm birth.

Objective: To compare the distribution and expression of hsp60 and hsp27 in placental trophoblast between preterm and term placenta and to observe hsp immune response in relation to the pathogenesis of preterm birth. Methods: 22 cases of preterm trophoblast, between 24 weeks and 36 weeks gestation, which were developed spontaneous onset or less than 24 hours after rupture of membrane were obtained. And aged-matched, 22 cases of normal term trophoblast, as control were also obtained after informed consent from each patient. The protein extraction form trophoblast was stained by immunohistochemical methods and was measured by the assay of Western blots. And the density of band using Image-writer were taken and statistical assay were performed as significance <0.05. Results: The expressions of hsp60 and hsp27 in trophoblast of preterm and term placenta were identified by immunohistochemical staining method. The hsp60 had significantly higher expression in trophoblast of preterm birth than in that of term birth (P<0.001) and the hsp27 also had significantly higher expression in trophoblast of preterm birth than in that of term birth (P<0.02) Conclusion: The higher expression of hsp60 and hsp27 in trophoblast of preterm birth might be suggested the development of immune response to occur preterm labor Further study are necessary to determine the exact actions of hsp60 and27 in trophoblast and to understand the immune mechanism of preterm birth.

Preterm birth, Heat shock protein (hsp), Trophoblast