Objective: The history of gestational diabetes (GDM) is a high risk for the development of type 2 diabetes mellitus (T2DM). The purpose of this study is to investigate the genetic association of LEP and LEPR gene polymorphisms and the development of T2DM in Korean women of history of GDM. Methods: Women diagnosed as GDM during pregnancy from January 1992 to December 2002 were recruited. Those women with a T2DM at the time of study were classified as T2DM positive group, and without T2DM, as T2DM negative group. 2 genes (LEP and LEPR genes) and 8 SNPs (LEP-632G>A, +4950G>A, +4998A>C, and LEPR-141013T>C, -186A>G, +5193G>A, +7187A>C, +27265A>G) were selected. The TaqMan assay for genotyping and the statistical analysis for phenotypic and genetic factors between 2 groups were analyzed. Results: A total of 54 women, T2DM positive (n=20) and T2DM negative (n=34) were enrolled. At the time of diagnosis of GDM, HbA1c, 50 g and 100 g oral glucose tolerance test, and insulin level were significantly associated between T2DM positive and negative groups (P<.05). In analysis of genetic risk to T2DM, the significant association related with any SNPs was not shown between T2DM positive and negative groups. Conclusion: In Korean women having past history of GDM, there was no relationship between 2 genes and the development to T2DM. To clarify a effect of candidate genes related with development of T2DM, there will need more samples and genes.

Objective: The history of gestational diabetes (GDM) is a high risk for the development of type 2 diabetes mellitus (T2DM). The purpose of this study is to investigate the genetic association of LEP and LEPR gene polymorphisms and the development of T2DM in Korean women of history of GDM. Methods: Women diagnosed as GDM during pregnancy from January 1992 to December 2002 were recruited. Those women with a T2DM at the time of study were classified as T2DM positive group, and without T2DM, as T2DM negative group. 2 genes (LEP and LEPR genes) and 8 SNPs (LEP-632G>A, +4950G>A, +4998A>C, and LEPR-141013T>C, -186A>G, +5193G>A, +7187A>C, +27265A>G) were selected. The TaqMan assay for genotyping and the statistical analysis for phenotypic and genetic factors between 2 groups were analyzed. Results: A total of 54 women, T2DM positive (n=20) and T2DM negative (n=34) were enrolled. At the time of diagnosis of GDM, HbA1c, 50 g and 100 g oral glucose tolerance test, and insulin level were significantly associated between T2DM positive and negative groups (P<.05). In analysis of genetic risk to T2DM, the significant association related with any SNPs was not shown between T2DM positive and negative groups. Conclusion: In Korean women having past history of GDM, there was no relationship between 2 genes and the development to T2DM. To clarify a effect of candidate genes related with development of T2DM, there will need more samples and genes.

목적: 임신성 당뇨 과거력의 여성이 산후 약 반수에서 제2형 당뇨병으로 발병하는 것으로 알려져 있다. 몇 가지 임상적 위험 인자가 제2형 당뇨병 발생에 관여하는 것으로 알려져 있으나 유전자와의 관련성에 대한 보고는 아직 없다. 본 연구는 leptin 및 leptin receptor 유전자가 임신성 당뇨의 과거력을 가진 여성에서 향후 제2형 당뇨병으로의 발병에 관여하는지 알아보고자 하는데 있다.연구방법: 1992년 1월 1일부터 2002년 12월 31일까지 산부인과에 내원하여 임신성 당뇨로 진단된 여성을 대상으로 하였다. 연구 시점에서 제2형 당뇨병으로 확인된 경우를 제2형 당뇨병 양성군, 확인이 안 된 경우를 제2형 당뇨병 음성군으로 정의하였다. 임신성 당뇨 진단시와 연구 시점에서의 임상적 자료를 분석하였다. 관련이 있는 유전자를 알아보기 위해 leptin 및 leptin receptor 유전자와 8개의 SNP (LEP-632G>A, +4950G>A, +4998A>C, and LEPR-141013T>C, -186A>G, +5193G>A, +7187A>C, +27265A>G)를 선별하였다. 두 군 간에 유전적 위험도를 분석하고 두 군간에 통계적으로 차이를 보이는 SNP가 있는지 조사하였다.결과: 임신성 당뇨 과거력을 가지고 있는 54명이 참여하였고, 제2형 당뇨병 양성군이 20명, 음성군이 34명으로 확인되었다. 임신성 당뇨 진단시 제2형 당뇨병 양성군과 음성군 간에 HbA1c와 50 g 및 100 g 당부하검사, 인슐린 농도 등이 통계적으로 유의하였다 (P<.05). 연구 시점에서 두 군간에 산과력 (P<.001) 외에는 통계적으로 유의한 차이가 없었다. 임신성 당뇨 진단 후 제2형 당뇨병 발생으로의 유전적 위험도 분석에서 두 군간에 유의한 차이를 보이는 SNP는 없었다.

제2형 당뇨병, 임신성 당뇨, Leptin 유전자, Leptin receptor 유전자, 단일 염기 다변형