목적: 만삭임신 태반에서의 배아성 줄기세포포지자의 부위별 발현을 비교하기 위하여 본 연구를 시행하였다. 연구 방법: 만삭임신의 태반에서 배아성 줄기세포 표면항원인 TRA 1-60 (Tumor rejection antigen 1-60), TRA 1-81 (Tumor rejection antigen 1-81), SSEA-3 (stage-specific embryonic antigen-3) 및 SSEA-4 (stage-specific embryonic antigen-4)에 대한 면역조직화학적 방법을 이용하여 태반의 부위별 항원 발현을 비교하였다. 결과: 양막상피세포는 TRA 1-60과 TRA 1-81에 대하여 양성반응을 보였고 양막중간엽기질세포는 TRA 1-81에 대하여 양성 반응을 보였다. 융모막중간엽기질세포는 TRA 1-60과 TRA 1-81에 대하여 양성반응을 보였다. SSEA-3와 SSEA-4는 태반의 어느 부위에서도 염색이 되지 않았다. TRA 1-60이 가장 강하게 발현된 부위는 양막상피세포였고 TRA 1-81이 가장 강하게 발현된 부위는 융모막중간엽기질세포였다. 결론: 만삭 태반에서의 양막상피세포 뿐 아니라 양막 및 융모막기질세포도 다능성 줄기세포의 유용한 자원이 될 수 있을 것으로 사료된다.

Objective: To determine which compartments of placenta in the term pregnancy express the embryonic stem cell markers. Methods: We have used immunohistochemical methods with antibodies to embryonic stem cell surface antigens, TRA 1-60 (Tumor rejection antigen 1-60), TRA 1-81 (Tumor rejection antigen 1-81), SSEA-3 (stage-specific embryonic antigen-3) and SSEA-4 (stage-specific embryonic antigen-4), to identify and localize stem cells in the term placenta. Results: Stem cell marker-positive cells were found in all layer of placenta. Amnionic epithelial cells was immunoreactive with TRA 1-60, TRA 1-81. Amnionic mesenchymal stromal cells was immunoreactive with TRA 1-81. Chorionic mesenchymal stromal cells was immunoreactive with TRA 1-60 and TRA 1-81. SSEA-3 and SSEA-4 were not stained at any compartment of the term placenta. Compartment that was stained most strongly by TRA 1-60 was the amnionic epithelial cells layer. Compartment that was stained most strongly by TRA 1-81 was the chorionic mesenchymal stromal cells layer. Conclusion: The mesenchymal stroma cells of the amnion and chorion as well as amnionic epithelial cells may be useful source of pluripotent stem cells in the term placenta.

Objective: To determine which compartments of placenta in the term pregnancy express the embryonic stem cell markers. Methods: We have used immunohistochemical methods with antibodies to embryonic stem cell surface antigens, TRA 1-60 (Tumor rejection antigen 1-60), TRA 1-81 (Tumor rejection antigen 1-81), SSEA-3 (stage-specific embryonic antigen-3) and SSEA-4 (stage-specific embryonic antigen-4), to identify and localize stem cells in the term placenta. Results: Stem cell marker-positive cells were found in all layer of placenta. Amnionic epithelial cells was immunoreactive with TRA 1-60, TRA 1-81. Amnionic mesenchymal stromal cells was immunoreactive with TRA 1-81. Chorionic mesenchymal stromal cells was immunoreactive with TRA 1-60 and TRA 1-81. SSEA-3 and SSEA-4 were not stained at any compartment of the term placenta. Compartment that was stained most strongly by TRA 1-60 was the amnionic epithelial cells layer. Compartment that was stained most strongly by TRA 1-81 was the chorionic mesenchymal stromal cells layer. Conclusion: The mesenchymal stroma cells of the amnion and chorion as well as amnionic epithelial cells may be useful source of pluripotent stem cells in the term placenta.

Placenta, Embryonic stem cell markers