목적 : Transforming growth factor-β는 Epithelial-mesenchymal transitions을 유발한다. Matrix Metallo- proteinases는 세포 이동을 유도하는 효소로 알려져 있다. 수정체 상피세포에서 EMT 중 세포 이동에 관여하는 MMPs의 종류와 조절 기전에 대해서 알아보고자 한다. 대상과 방법 : 수정체 상피세포의 EMT 모델로 rat의 lens explant culture를 이용하였으며. 핵 백내장과 전낭하 백내장 환자의 수정체 낭을 사용하였다. 면역 형광 염색법과 RT-PCR을 통해 MMPs과 Src family kinase의 발현을 확인하였다. 결과 : TGF-β를 처리한 explant에서는 MMP-2와 -14의 발현이 증가하였다. 반면 전낭하 백내장에서는 MMP-14의 발현이 증가하였다. explant에서 이 두 MMPs들은 모두 SFKs의 억제제인 PP2에 의해 억제되었으며, 전낭하 백내장에서도 SFKs가 활성화되어 있음을 확인하였다. 결론 : TGF-β에 의한 수정체 상피세포의 이동에서 SFKs에 의해 조절되는 MMP-2와 -14가 중요한 역할을 할 것으로 생각된다.

Purpose: TGF-β is a key regulator of epithelial-mesenchymal transition. Among the TGF-β responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-β and examined the TGF-β signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) Methods: First we examined the expression of MMPs in rat lens explant culture treated with TGF-β and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. Results: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. Conclusions: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-β.

Purpose: TGF-β is a key regulator of epithelial-mesenchymal transition. Among the TGF-β responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-β and examined the TGF-β signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) Methods: First we examined the expression of MMPs in rat lens explant culture treated with TGF-β and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. Results: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. Conclusions: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-β.

Lens epithelial cell, Matrix metalloproteinase, Nuclear and anterior polar cataract, Transforming growth factor-