목적 : polyethylene glycol (PEG)의 표면 중합이 후낭 혼탁 발생에 미치는 효과를 연구하고자 하였다. 대상과 방법 : PEG로 표면 중합된 아크릴렌즈(PEG-IOL)와 대조군을 이용하여 사람수정체 상피세포주를 1 ml당 1 ×104를 각각의 표면 위에 분주하였다. CO2 조건하에서 24시간 동안 배양하고 trypsin 처리 후 tryphan blue로 염색을 하여 세포의 부착 정도를 살폈다. 또한 20마리의 토끼에 각군의 렌즈를 삽입하고 후낭혼탁의 형성 정도를 역반사 조명으로 기록한 후 POCOman프로그램을 이용하여 비교하였다. 2개월 후 렌즈를 적출하고 주사 전자 현미경으로 렌즈 표면에 부착된 세포의 성상을 살폈다. 결과 : 부착된 세포는 대조군 3.6±1.9×103, PEG-IOL 3.2±1.1×103으로 통계적으로 유의하지는 않지만 PEG-IOL에서 적었다. 형성된 후낭 혼탁은 관찰 3주째 PEG-IOL에서 대조군에 비해 유의하게 감소된 점이 관찰되었고 주사현미경에서 세포수의 차이는 없었으나 형태적인 차이가 관찰되었다. 결론 : PEG의 표면 중합은 인공수정체 삽입 후 발생하는 후낭 혼탁을 줄일 수 있는 가능성이 있을 것으로 생각된다. <한안지 47(4):621-628, 2006>

Purpose: To investigate the effect of polyethylene glycol (PEG)-grafted acryl intraocular lenses on the prevention of posterior capsular opacification (PCO). Methods: The acrylic surface of an intraocular lens (Acrysof SA 60AT, Alcon) was polymerized with PEG (PEG-IOL). To investigate the degree of cell adhesion to the modified lens surface, human lens epithelial cells (1×104 cells/ml) were inoculated on each PEG-grafted and acrylic control lens, and all were cultured in a carbon dioxide incubator for 24 hours. The adhered cells were trypsinized and counted. The PEG-IOL was implanted in 20 New Zealand rabbits after removal of the crystalline lens. The formations of PCO were checked serially through retroilluminated digital photography and severity scores were calculated using POCOman. The cell adherence pattern on the PEG-grafted IOL was examined by scanning electron microscopy. Results: The mean number of adherent cells in PEG-IOL was 3.2±1.1×103, which tended to be smaller than that of the unmodified acrylic control (3.6±1.9×103), but without statistical significance. The mean severity of posterior capsular opacification in PEG-IOL was much lower than in the control, especially at week 3. Scanning electron microscopy revealed more patch-like cells firmly attached to the lens surface in the control. Conclusions: PEG polymerization of the acrylic IOL may lessen the formation of posterior capsular opacification. J Korean Ophthalmol Soc 47(4):621-628, 2006

Purpose: To investigate the effect of polyethylene glycol (PEG)-grafted acryl intraocular lenses on the prevention of posterior capsular opacification (PCO). Methods: The acrylic surface of an intraocular lens (Acrysof SA 60AT, Alcon) was polymerized with PEG (PEG-IOL). To investigate the degree of cell adhesion to the modified lens surface, human lens epithelial cells (1×104 cells/ml) were inoculated on each PEG-grafted and acrylic control lens, and all were cultured in a carbon dioxide incubator for 24 hours. The adhered cells were trypsinized and counted. The PEG-IOL was implanted in 20 New Zealand rabbits after removal of the crystalline lens. The formations of PCO were checked serially through retroilluminated digital photography and severity scores were calculated using POCOman. The cell adherence pattern on the PEG-grafted IOL was examined by scanning electron microscopy. Results: The mean number of adherent cells in PEG-IOL was 3.2±1.1×103, which tended to be smaller than that of the unmodified acrylic control (3.6±1.9×103), but without statistical significance. The mean severity of posterior capsular opacification in PEG-IOL was much lower than in the control, especially at week 3. Scanning electron microscopy revealed more patch-like cells firmly attached to the lens surface in the control. Conclusions: PEG polymerization of the acrylic IOL may lessen the formation of posterior capsular opacification. J Korean Ophthalmol Soc 47(4):621-628, 2006

Acrylate, Intraocular lens, Polyethylene glycols, Posterior capsular opacity