목적 : 가토에서 실험적 국소 망막박리가 박리된 망막과 박리 주변 유착 망막에 미치는 영향과 유리체내 트리암시놀론 주입이 망막박리, 특히 뮐러세포의 신경아교증(gliosis)에 미치는 영향을 알아보고자 하였다. 대상과 방법 : 유색 가토 12마리를 대상으로 양눈에 유리체절제술을 시행한 후, 망막하로 히알루론산을 주사하여 국소 망막박리를 유발시킨 후 한눈에만 트리암시놀론 5 mg을 유리체내로 주사하고 3일, 7일 그리고 28일째에 각각 4마리씩 안구 적출하여 박리된 망막과 주변 망막의 조직검사와 전자현미경검사, 그리고 뮐러세포에 대한 면역조직화학염색을 실시하였다. 결과 : 망막박리는 박리 주변 유착 망막에도 변성을 유발하며 특히, 광수용체와 신경절세포의 변성이 관찰되었고, 박리된 망막의 두께는 감소되었으나 망막내층의 두께는 비교적 유지되었다. 또한 신경아교증이 박리된 망막과 주변 유착 망막에서도 증가되어 있었다. 하지만 유리체내 트리암시놀론은 이러한 망막변성과 망막두께 그리고 뮐러세포의 신경아교증에는 영향을 미치지 않았다. 결론 : 국소 망막박리는 박리된 망막과 주변 유착 망막의 조직학적 변성을 유발하며, 이는 망막 재유착술 후 확대된 시야장애에 대한 원인으로 생각할 수 있다.

Purpose: To investigate the effect of localized retinal detachment on both the detached and attached regions, and to determine the effect of triamcinolone on Müller cell gliosis. Methods: Pars plana vitrectomy was performed in both eyes of 12 pigmented rabbits. A dome‐shaped retinal detachment was made by injecting sodium hyaluronate into the subretinal space. Triamcinolone (5 mg) was applied intravitreally to one eye (12 eyes). The detached retinal area and the neighboring attached region were studied by light and electron microscopy 3, 7, and 28 days after surgery. Tissues were prepared in 5 um sections for hematoxylin-eosin staining and immunohistochemistry with antibody to glial fibrillary acidic protein (GFAP). Results: In addition to the well-known degeneration of photoreceptor cells in the detached retina, an incomplete but severe loss of ganglion cell axons occurs in both the detached and the attached regions. The total retinal thickness gradually decreased in the detached areas, while the thickness of the inner retinal layers remained virtually unchanged over several weeks. Gliotic alterations were apparent in both the detached and non-detached retinal areas, and intravitreal triamcinolone did not alter these gliotic alterations of Müller cells. Conclusions: It is noteworthy that progressive retinal destruction also occurs in the attached retina after local detachment. This may account for visual impairment in strikingly large areas of the visual field, even after retinal reattachment.

Purpose: To investigate the effect of localized retinal detachment on both the detached and attached regions, and to determine the effect of triamcinolone on Müller cell gliosis. Methods: Pars plana vitrectomy was performed in both eyes of 12 pigmented rabbits. A dome‐shaped retinal detachment was made by injecting sodium hyaluronate into the subretinal space. Triamcinolone (5 mg) was applied intravitreally to one eye (12 eyes). The detached retinal area and the neighboring attached region were studied by light and electron microscopy 3, 7, and 28 days after surgery. Tissues were prepared in 5 um sections for hematoxylin-eosin staining and immunohistochemistry with antibody to glial fibrillary acidic protein (GFAP). Results: In addition to the well-known degeneration of photoreceptor cells in the detached retina, an incomplete but severe loss of ganglion cell axons occurs in both the detached and the attached regions. The total retinal thickness gradually decreased in the detached areas, while the thickness of the inner retinal layers remained virtually unchanged over several weeks. Gliotic alterations were apparent in both the detached and non-detached retinal areas, and intravitreal triamcinolone did not alter these gliotic alterations of Müller cells. Conclusions: It is noteworthy that progressive retinal destruction also occurs in the attached retina after local detachment. This may account for visual impairment in strikingly large areas of the visual field, even after retinal reattachment.