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Little research has been done regarding the issues of Ecotourists' behaviour on visiting Eco-tourism sites. Thus, this study investigated how tourist' motivation and expectation of visiting Upo wetland influence their satisfaction and behaviour. The key variables of this study are 1) motivation, satisfaction, expectation, behaviour intention of tourist before/after traveling.the analysis of this study, SPSS 12.0 was used which included was used factor analysis, descriptive analysis and multiple regression analysis.findings of this study suggest as follows. Firstly, although tourists showed their visit expectations for preservation of natural environment and value of high living. They had low intention participation of environmental education programmes. Secondly, tourists' motivation influenced tourists' satisfaction and intention of behaviour in general by visit. Particularly, posttravel survey showed that motivation of learning and enjoying natural environmenthad an impactsatisfaction.Finally, those tourists who had experience of environmental education, and environment tour before the survey showed more satisfaction of tourism. Furthermore, the post survey showed that their experience environmental tour affected their value of benefits of environmental tour as well as environmental preservation and rest.