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The aim of this study is to propose some conditions for ‘Eco-space’ in the city. For that reason, we interpreted the hidden meaning of ‘Eco-space’ of Nonhyun area in Incheon metropolitan city with semiotic method; how to be represented ecological meaning on that space by ‘Eco-concept’. The ‘Eco-space’ is one of conditions for improving the quality of life in the city. It is constructed by the ‘Eco-concept’. The definition is an idea or abstract principle for the reification of meaning of ‘the coexistence of people with Nature’. In the macro point of view, the ‘Eco-space’ is the ‘environment to which people adapt oneself’. In the micro point of view, the ‘Eco-space’ is the ‘environment with which people cooperate’. This is almost filled of symbolic signs(artificial things), but they change symbolic to iconic ones. The ‘Eco-space’ of the connection is balanced Nature of original ecosystem and the benefit of people. The consequence of the semiotic interpretation is following. There is the kind of role between ‘the meaning of Eco-space from the relation between people and Nature’ and ‘the nature of signs on Eco-space’. It is caused by balancing the benefit of Nature and people. And it explains both of ‘natural eco-space’ and ‘artificial eco-space’, ‘Natural eco-space’ is filled of ecological icons and remains original Nature. So it is ‘environment to which people adapt oneself’, ‘the space of cultural Nature’ and symbolization. ‘Artificial eco-space’ is filled of ecological symbols and made up through the aim for ‘Eco-space’. So it is ‘environment with which people cooperate’, ‘the space of natural culture’ and iconization.