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This study was conducted to investigate the effects of organizational culture on organizational commitment and intention of turnover in hotel. From the literature review, the organizational culture measurement tool of hotel employees has been developed. In addition, an empirical study has been conducted to identify the relationships among 4 typical factor organizational culture, organizational commitment, and turnover intention in hotel. To test each measures' predictive utility in this context with AMOS. As the result, developmental culture and group culture are major to influence on the organizational commitment, while hierarchical culture and rational culture aren't determinant to influence on the organizational commitment. Also, organizational commitment are negative major to influence on the turnover intention.