신생흰쥐의 심장근육조직에서 최초의 혈관형성의 형태와 시기를 추적하기 위해 중간엽세포가 혈관내피 세포로 분화될 때 합성분비하는 von Willebrand factor (vWf)의 활성을 관찰하고자 하였다. 출생후 1일부터 7일까 지의 신생흰쥐를 실험동물로 사용하였으며, 심장조직을 전체를 동결하여 14~20 µm 두께의 동결절편을 제작하고 vWf를 추적하기 위해 goat anti-von Willebrand factor와 biotinylated rabbit anti-goat IgG를 사용하여 면역염색을 하여 다음과 같은 결과를 얻었다. 1. 출생 제 1일의 신생흰쥐의 심장조직에서 심장속막 및 바깥막의 세포밖바탕질에 중등도의 vWf 면역반응이 나타 났고 심장근육층의 세포밖바탕질에는 약한 반응을 보였다. 2. 출생 제 2일의 신생흰쥐 심장조직에 처음으로 소수의 혈섬이 형성되고 출생 제 4일의 심장의 대부분에서 혈섬 이 형성되었으며 세포밖바탕질에도 중등도의 vWf 면역반응이 나타났다. 3. 출생 제 7일의 심장근육층에 소수의 혈관이 형성되어 있었고 혈관내피세포와 세포밖바탕질에 중등도의 vWf 면 역반응이 관찰되었다. 이상과 같은 출생 초기의 심장조직은 중간엽세포에서 혈섬과 심장근육세포로 분화되면서 세포밖바탕질내 vWf 분 포가 증가되고 이때 혈관 형성이 시작되는 것으로 생각되었다.

To investigate the process of angiogenesis in the cardiac tissue of neonatal rats, the levels of von Willebrand factor (vWf) produced by endothelial cells were observed. At days 1 to 7 after birth, whole heart obtained from neonatal rats was frozen sectioned, stained with anti-vWf and biotinylated rabbit anti-goat IgG antibodies, followed by immunohistochemical examinations. The results were as follows: 1. At day 1 after birth, extracellular matrix of endocardium and epicardium was stained with anti-vWf at the intermediate level, but that of myocardium was at the low level. 2. At day 2 after birth, a few blood islands were detected. At day 4 after birth, blood island was formed in most parts of heart and extracellular matrix was stained with ant-vWf at the intermediate level. 3. At day 7 after birth, a few blood vessels were formed, and endothelial cells and extracellular matrix was stained with ant-vWf at the intermediate level. These results suggest that mesenchymal cells were differentiated to blood islands and myocardiac cells, which are responsible for the distribution of vWf in extracellular matirx and for angiogenesis.

To investigate the process of angiogenesis in the cardiac tissue of neonatal rats, the levels of von Willebrand factor (vWf) produced by endothelial cells were observed. At days 1 to 7 after birth, whole heart obtained from neonatal rats was frozen sectioned, stained with anti-vWf and biotinylated rabbit anti-goat IgG antibodies, followed by immunohistochemical examinations. The results were as follows: 1. At day 1 after birth, extracellular matrix of endocardium and epicardium was stained with anti-vWf at the intermediate level, but that of myocardium was at the low level. 2. At day 2 after birth, a few blood islands were detected. At day 4 after birth, blood island was formed in most parts of heart and extracellular matrix was stained with ant-vWf at the intermediate level. 3. At day 7 after birth, a few blood vessels were formed, and endothelial cells and extracellular matrix was stained with ant-vWf at the intermediate level. These results suggest that mesenchymal cells were differentiated to blood islands and myocardiac cells, which are responsible for the distribution of vWf in extracellular matirx and for angiogenesis.

: Neonate of rat, Angiogenesis, von Willebrand factor, Heart