말초신경병증에서 운동이 뼈대근육 재생에 미치는 영향에 대해 논란이 많다. 본 연구의 목적은 흰쥐의 궁둥신경에 압궤손상을 준 뒤 일정기간 트레드밀에서 달리기운동을 시켜 운동이 근육재생에 효과가 있는지를 밝힐 목적으로 시행하였다. 1개월(체중 150~180 g)된 수컷 Sprague-Dawley계 흰쥐의 궁둥신경을 노출시킨 다음 지혈집게로 3분간 압궤손상을 주었다. 각 실험군 별로 하루 60분 씩 주 5회 운동을 시켰으며, 트레드밀의 경사도는 10도, 속도는 20 m/분이었다. 궁둥신경 압궤손상 후 12일(5일간 운동), 19일(10일간 운동), 26일(15일간 운동), 33일(20일간 운동) 및 61일(40일간 운동)에 흰쥐를 희생시켜 장딴지근을 절취하여 냉동절편을 만들었으며 근육섬유에 대한 느린 미오신(slow myosin) 면역조직화학적 염색과 NADH-TR 효소조직화학 염색을 통상적인 절차에 따라 시행하였다. 압궤손상 후 12일째와 19일째에서 실험군의 흰쥐들이 대조군의 흰쥐보다 근육섬유의 위축이 심하였고, 33일째에서 실험군의 흰쥐들은 각진 모양의 근육 섬유들이 관찰되었으며, 61일째에서 정상근육에 가깝게 회복되었으나 대조군의 흰쥐는 그렇지 못했다. 압궤손상 후 26일째 운동군에서 II형 섬유의 중심에서 재신경지배가 되는 것이 관찰되었다. 손상 후 33일째에는 거대한 II형 섬유들이 관찰되었고, 재신경지배근육의 특성이라 할 수 있는 동일 근육섬유 군집현상이 나타났고, 61일째에는 정상근육에 가까운 근육섬유의 조성비를 보였다. 대조군에서는 손상 후 33일째에 거대한 II형 섬유들이 보이기 시작하였으나, 61일째에는 정상근육에 이르지는 못하였다. 이상의 결과로 흰쥐의 궁둥신경에 압궤손상을 준 후 트레드밀을 이용한 달리기 운동은 근육재생에 효과적인 도움을 준다고 생각된다.

It is constant controversy that exercise influence muscle regeneration in peripheral neuropathy. The aim of this experiment is to show that treadmill running exercise under well-controlled conditions is to improve of regeneration in rat gastrocnemius muscles after sciatic nerve crushing injury. Male Sprague-Dawley rats (1 month old, weight 150~180 g) were submitted to bouts of exercise on a treadmill up a 10 degrees decline and speed is 20 m/min for 60 min per day and gastrocnemius muscles were analysed at different exercise periods (5, 10, 15, 20 and 40 days) by immunohistochemistry in comparison with injured non-exercised muscles. Rats were sacrificed at 12th (5 days exercise), 19th (10 days exercise), 26th (15 days exercise), 33rd day (20 days exercise), 61st day (40 days exercise) after sciatic nerve crushing injury. It showed that type II myofibers (target fibers) on center area had reinnervation at sciatic nerve crush injury at 26th day in exercise rats, as at 33rd day appeared giant type II myofibers, myofibers grouping observed in regenerative muscle character, component ratio of closed normal muscle showed at 61st day. Giant type II myofibers showed at 33rd day in non-exercise rats, however did not nearly normal muscle at 61st day. Therefore we concluded that treadmill running exercise is able to improve regeneration processes in gastrocnemius muscles after sciatic nerve crushing injury of rats.

It is constant controversy that exercise influence muscle regeneration in peripheral neuropathy. The aim of this experiment is to show that treadmill running exercise under well-controlled conditions is to improve of regeneration in rat gastrocnemius muscles after sciatic nerve crushing injury. Male Sprague-Dawley rats (1 month old, weight 150~180 g) were submitted to bouts of exercise on a treadmill up a 10 degrees decline and speed is 20 m/min for 60 min per day and gastrocnemius muscles were analysed at different exercise periods (5, 10, 15, 20 and 40 days) by immunohistochemistry in comparison with injured non-exercised muscles. Rats were sacrificed at 12th (5 days exercise), 19th (10 days exercise), 26th (15 days exercise), 33rd day (20 days exercise), 61st day (40 days exercise) after sciatic nerve crushing injury. It showed that type II myofibers (target fibers) on center area had reinnervation at sciatic nerve crush injury at 26th day in exercise rats, as at 33rd day appeared giant type II myofibers, myofibers grouping observed in regenerative muscle character, component ratio of closed normal muscle showed at 61st day. Giant type II myofibers showed at 33rd day in non-exercise rats, however did not nearly normal muscle at 61st day. Therefore we concluded that treadmill running exercise is able to improve regeneration processes in gastrocnemius muscles after sciatic nerve crushing injury of rats.

Sciatic nerve crushing injury, Muscle regeneration, Treadmill exercise, Myosin, NADH-TR